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. 2018 Nov 27;19(12):3767. doi: 10.3390/ijms19123767

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expressions of exogenous oct4, sox2, klf4, and myc in BV-pr-OSKM-transduced ZF4 cells. (A) Structure of pFastBac-pr-OSKM. Driven by a CMV promoter, oct4 and sox2 were fused by a 2A peptide, which was similar in klf4 and myc. (B–D) ZF4 cells transduced with BV-pr-OSKM. Scale bars, 200 μm. (E) cDNA fragments of oct4 (400 bp), klf4 (399 bp), sox2 (385 bp), myc (312 bp), β-actin (382 bp), and pr (221 bp) were detected by semiquantitative RT-PCR in ZF4 cells transduced with BV-pr-OSKM or control BV-CMV-ie1-pr (BV-pr). M, DNA marker; oct4, octamer-binding transcription factor 4; sox2, sex determining region Y-box 2; klf4, Kruppel-like factor 4; myc, homology with the Avian viral gene v-myc.