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. 2018 Nov 27;19(12):3767. doi: 10.3390/ijms19123767

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The activity of the WSSV ie1 promoter in medaka embryos. (A) Rough sketch of pCMV-H2B-Cherry and pFastBac-CMV-ie1-pr that were used for microinjection into fish embryos. In pCMV-H2B-Cherry, a H2B-cherry cassette was driven by a CMV promoter, and the H2B polypeptide was a nuclear localization signal. In pFastBac-CMV-ie1-pr, a pr cassette was driven by a WSSV ie1 promoter. A, poly(A). (B–G) The RFP expressions in medaka embryos where the plasmids pCMV-H2B-Cherry or pFastBac-CMV-ie1-pr were injected. Scale bar, 500 μm.