Figure 5.

Influence of cinnamaldehyde-related compounds on the cytosolic pro-IL-1β, NLRP3 and pro-caspase-1 levels in macrophages stimulated with LPS and ATP. The panels display a representative photogram of Western blot (a) and the quantified results of pro-IL-1β (b), NLRP3 (c) and pro-caspase-1 (d), separately. The macrophages were pretreated with 100 μM cinnamaldehyde-related compounds for 30 min and then treated with LPS and ATP. Cytosolic proteins were isolated and the pro-IL-1β, NLRP3 and pro-caspase-1 amounts were further detected by Western blot. Data are expressed as the mean ± SD and different superscript letters represent statistically differences (p < 0.05).