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Effect of kisspeptin-10 (100 nM–10 μM) treatment on extracellular dopamine (DA), norepinephrine (NE), and serotonin (5-hydroxytriptamine, 5-HT) levels, as determined by HPLC in rat hypothalamic (Hypo-E22) cell line (n = 5 individual culture flasks for each condition). Compared to control, kisspeptin-10 treatment significantly decreased extracellular DA (ANOVA, p < 0.01; post-hoc, ** p < 0.01 vs. control) and 5-HT (ANOVA, p < 0.01; post-hoc, ** p < 0.01 vs. control) levels.
