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Effect of kisspeptin-10 (100 nM–10 μM) treatment on extracellular dihydroxyphenylacetic acid (DOPAC)/dopamine (DA) and 5-hydroxyindoleacetic acid (5-HIIA)/serotonin (5-HT) ratios as determined by HPLC in rat hypothalamic (Hypo-E22) cell line (n = 5 individual culture flasks for each condition). Compared to control, kisspeptin-10 treatment significantly increased extracellular DOPAC/DA and 5-HIIA/5-HT ratios (ANOVA, p < 0.01; post-hoc, * p < 0.05 vs. control).
