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The typical HPLC chromatographic profile of mixed standards and total biflavonoid extract acquired in five extraction methods. (a) Heat-reflux extraction; (b) Soxhlet extraction; (c) percolation extraction; (d) ethanol-UAE; (e) IL-UAE; (f) mixed standards. The peaks marked with 1, 2, 3, and 4, which were AME, HIN, GIN, and HEV, respectively.
