Table 1.

Particle size, polydispersity index, and zeta potential of (a) β-Sit-PLGA and β-Sit-PEG-PLA nanoparticles, and (b) coumarin-6-labeled nanoparticles. Results represent mean ± SD (n = 3).

(a)
Formulation	Weight of β-sitosterol Used for Formulation
	2 mg/mL			4 mg/mL
	Z-average (nm)	PDI	ζ (mV)	Z-average (nm)	PDI	ζ (mV)
β-Sit-PLGA-EtAC	215.1 ± 29.7	0.10 ± 0.003	−13.9 ± 1.61	231.2 ± 0.60	0.126 ± 0.02	−16.1 ± 7.09
β-Sit-PLGA-DCM	311.1 ± 94.4	0.242 ± 0.03	−14.0 ± 6.01	261.6 ± 4.74 a	0.113 ± 0.03 a	−16.5 ± 0.21 a
β-Sit-PEG-PLA (R45)	240.6 ± 23.3	0.18 ± 0.05	−23.5 ± 0.27	276.7 ± 1.01	0.197 ± 0.01	−21.5 ± 6.56
β-Sit-PEG-PLA (R25)	239.5 ± 7.9	0.17 ± 0.05	−24.5 ± 0.99	279.4 ± 0.85	0.112 ± 0.04	−21.2 ± 7.01
(b)
Formulation	Physicochemical Properties of Coumarin 6-Labeled Nanoparticles
	Z-average (nm)		PDI		ζ (mV)
β-Sit-PLGA-EtAC	228 ± 0.45		0.10 ± 0.01		−16.8 ± 0.33
β-Sit-PEG-PLA (R45)	245 ± 0.66		0.09 ± 0.02		−19.6 ± 0.22

^a Result represents mean ± SD (n = 2); ζ zeta potential.