Figure 5.

Effects of heterologous VqJAZ7 expression in transgenic Arabidopsis on B. cinerea symptoms and defense-related enzyme activity. VqJAZ7 transgenic lines (TL1, TL2, and TL3) and nontransgenic controls (Col-0) were infected with B. cinerea (A) Images of plants at 72 h post-inoculation (hpi). Trypan blue and DAB staining were carried out on leaves 72 hpi in order to detect cell death (B); and H₂O₂ (C), respectively. Scale bars = 10 mm; (D) Botrytis average lesion diameter at 72 hpi; (E) Symptoms on transgenic and control lines 72 hpi with B. cinerea. Activity of catalase (CAT) (F); peroxidase (POD) (G) and superoxide dismutase (SOD) (H) in the leaves of transgenic and WT plants. Asterisks indicate significant difference between nontransgenic (NT) and transgenic lines (**: p < 0.01, *: p < 0.05, Student’s t-test).