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. 2018 Nov 22;19(12):3716. doi: 10.3390/ijms19123716

Figure 1.

Figure 1

AMP-activated protein kinase (AMPK) is required for the expression of immediate early genes (IEGs) following glutamatergic activation. (a) Primary neurons at 15 days in vitro (DIV) treated with bicuculline and 4-aminopyridine (Bic/4-AP) (50µM/2.5mM) for the indicated time were subjected to immunoblotting with anti- phospho-AMPK (pAMPK), phospho-acetyl-CoA carboxylase (pACC), acetyl-CoA carboxylase (ACC), total AMPK, Arc, c-Fos, EgrI, and actin antibodies. Results are representative of at least four experiments. (b) Quantification of Western blot (WB) as in (a) showing Arc, c-Fos, and EgrI expression. Results show mean ± SD (n = 4). One-way ANOVA followed by Bonferroni’s post-hoc test were used for evaluation of statistical significance, * p < 0.05, *** p < 0.001 compared to control condition. (c) Primary neurons at 15 DIV were pre-treated for 20 min in presence or absence of the AMPK inhibitor Compound C (Cc, 10 µM) prior to being treated with Bic/4-AP (50µM/2.5mM, 2 h). Cell lysates were subjected to immunoblotting with anti-Arc, c-Fos, EgrI, and actin antibodies. Results are representative of at least four experiments.