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. 2018 Dec 4;19(12):3867. doi: 10.3390/ijms19123867

Figure 7.

Figure 7

The −1 frameshifting is not required for +1 frameshifting attenuation activity of mPK. (A) SDS-PAGE analysis of in vitro radioactivity-based frameshifting assay of constructs containing CUUUGA frameshifting site with an UAU codon in the E-site, an upstream internal SD of dnaX (SD0), and different downstream mPK variants (WT and MT). The values displayed are means ± SD of three independent experiments. (B) Relative +1 PRF activity of (B) with asterisks indicating p value < 0.001 using Student’s t-test. (C) SDS-PAGE analysis of in vitro radioactivity based frameshifting assay of a construct with an A6G frameshifting site and a stable upstream H1 hairpin. A5G is a read-through control with the A6G slippery sequence mutated. The values displayed are means ± SD of three independent experiments.