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. 2018 Nov 27;23(12):3101. doi: 10.3390/molecules23123101

Figure 2.

Figure 2

Silencing of EGFP gene expression in BHK IR780 cells by siEGFP delivered by cationic liposomes in serum-free conditions (A) or in the presence of 10% FBS (B). The cells were incubated with the lipoplexes formed by cationic liposomes (■ for L, ▲ for P800, ● for P1500 and □ for P2000) and siEGFP (50 nM) at various N/P ratios. The levels of EGFP expression in the cells were evaluated using flow cytometry after 72 h of incubation with the lipoplexes. Levels of EGFP expression in the control cells (without any treatment) was set at 100%. The differences between mean values for L and other preparations at the same N/P were considered statistically significant: * p < 0.01 (Mann-Whitney U-test). siEGFP delivery by Lipofectamine 2000 (♦ for LF2000) (under optimal conditions) was used as a positive control.