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. 2018 Dec 6;19(12):3903. doi: 10.3390/ijms19123903

Figure 2.

Figure 2

Effect of Ber, Res and their combination on the lipid accumulation in adipocytes. Differentiated 3T3-L1 adipocytes were established by inducing for 10 days (details indicated in Material and Methods) and then treated with different concentration of Ber (B–D: 2, 12, 20 µmol/L), Res (E–F: 10, 25 µmol/L) and their combination (G–H: 12 µmol/L Ber + 25 µmol/L Res, 20 µmol/L Ber + 25 µmol/L Res) for 3 days. Control adipocytes were treated with the vehicle (DMSO < 0.1%) (A). Lipid droplets in adipocytes were detected by oil red O staining. The magnification of Microscope for observation was 10 (I) the absorbance density of Oil red dye extracted from differentiated adipocytes after Ber and Res treatment. *** p < 0.001, ** p < 0.01, * p < 0.05 vs. control, # p < 0.05 vs. single drug. Data were presented as mean ± SD from three separate experiments.