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Silver-stained SDS-PAGE of pRBC saponin extracts run through four affinity chromatography columns where AGMA1, ISA1, ISA23 or ARGO7 had been immobilized. (A) A RBC extract was first loaded, and after the corresponding washing-elution-washing steps (B) a pRBC extract was loaded in the same column. The approximate masses (kDa) of the seven bands from the molecular weight marker are indicated in the space between the gels.
