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Direct delivery of PAAs to Anopheles. Newly hatched A. atroparvus were fed fluorescein-labeled ISA1 incorporated in their sugar meal. Confocal fluorescence microscopy indicated the presence of fluorescein signal (A) in the midgut (first day post-feeding) and (B) in a region of the thorax consistent with salivary gland location (three days post-feeding). In red is shown the reflection of excitation light on the cuticle of the insect.
