Figure 5.

Network analysis of select miRNAs and their target mRNAs, as well as their impact on the disease process in multiple sclerosis. IPA-based schematic representation of various mRNA targets affected by the three major miRNAs altered during EAE development, namely miR-99b, miR-125a, and miR-146b, leading to differential effect on pathogenic versus protective T cell subset as well as neuroprotection versus neurodegeneration. The known interactions between the genes are represented by lines showing activation (arrow) or inhibition (blunt end). Further, solid line indicates direct interaction, whereas dashed line indicates indirect interaction. Colored lines indicate the following: orange line for activation, blue line for inhibition, yellow line for uncertain state of the downstream molecule, and gray line for effect not predicted. For the target genes, orange indicates predicted activation, whereas blue indicates predicted inhibition.