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. 2018 Dec 10;19(12):3970. doi: 10.3390/ijms19123970

Figure 2.

Figure 2

Effects of ClF (IC50) and EGCG (10 µM) or genistein (10 µM) alone and in combination on the number of alive, apoptotic and necrotic cells as well as on caspase 3 activity (only in MDA-MB-231 cells) in MCF7 (A,B) and MDA-MB-231 (C,D) cell lines. The original FACS cytograms of annexin V/propidium iodide and caspase 3 (only in MDA-MB-231 cells) assays for MCF7 (B) and MDA-MB-231 (D) cells are shown. Values are means ± S.D. of three independent experiments. Exposure versus vehicle control: *** p < 0.001, ** p < 0.01, * p < 0.05. Ct0, control cells used for experiments (time 0 h); Control (unexposed), negative control, cells incubated with medium; DAC, positive control; DMSO, vehicle control; Q1, necrotic cells (Ann−/PI+); Q2, late apoptotic cells (Ann+/PI+); Q3, viable cells (Ann −/PI−); Q4, early apoptotic cells (Ann+/PI−).