Effects of ClF (IC50) and EGCG (10 µM) or genistein (10 µM) alone and in combination on expression (left panel) and methylation (right panel) of PTEN tumor suppressor gene in MCF7 (A) and MDA-MB-231 (B) cells. All results represent mean ± SD of three independent experiments. Exposure (ClF alone, EGCG alone, genistein alone, ClF + EGCG or ClF + Genistein) versus vehicle control, *** p < 0.001, ** p < 0.01, * p < 0.05. EGCG or genistein alone versus combinatorial exposure (ClF + EGCG or ClF + Genistein), ^^ p < 0.01, ^ p < 0.05. ClF alone versus combinatorial exposure (ClF + EGCG or ClF + Genistein), ## p < 0.01, # p < 0.05.