Table 1.
Effects of ClF at IC50 concentration (640 nM and 50 nM in MCF7 and MDA-MB-231 cells, respectively), EGCG or genistein at 10 µM concentration, as well as ClF (IC50) in combination with EGCG or genistein (10 µM) on MCF7 and MDA-MB-231 cell viability ((viable exposed/viable vehicle control)*100%).
| A | Cell Viability | |||
| MCF7 | MDA-MB-231 | |||
| ClF (IC50) | 53.6 ± 2.5 *** | 54.2 ± 2.7 *** | ||
| EGCG 10 µM | 87.2 ± 7.6 * | 83.3 ± 8.9 * | ||
| Genistein 10 µM | 79.7 ± 8.1 * | 78.3 ± 13.0 * | ||
| ClF + EGCG | 37.1 ± 3.1 ***^^^## | 45.2 ± 2.7 ***^^# | ||
| ClF + Genistein | 39.9 ± 1.1 ***^^### | 44.9 ± 3.6 ***^# | ||
| B | MCF7 | MDA-MB-231 | ||
| ClF (IC50) | + | + | + | + |
| EGCG 10 µM | + | − | + | − |
| Genistein 10 µM | − | + | − | + |
| Average CI | 0.8021 ± 0.0458 *** | 1.1439 ± 0.0913 | 0.7825 ± 0.0593 ** | 1.0808 ± 0.1065 |
(A) All results represent mean ± SD of three independent experiments. Exposure (ClF alone, EGCG alone, genistein alone, ClF + EGCG, or ClF + Genistein) versus vehicle control, *** p < 0.001, ** p < 0.01, * p < 0.05. EGCG or genistein alone versus combinatorial exposure (ClF + EGCG or ClF + Genistein), ^^^ p < 0.001, ^^ p < 0.01, ^ p < 0.05. ClF alone versus combinatorial exposure (ClF + EGCG or ClF + Genistein), ### p < 0.001, ## p < 0.01, # p < 0.05. (B) CompuSyn data of trypan blue exclusion test values [57] (cell growth inhibition, (viable exposed/viable vehicle control)) indicate synergistic (CI < 1) effect of ClF + EGCG and nearly additive (CI = 1) effect of ClF + Genistein in both MCF7 and MDA-MB-231 breast cancer cells. CI, combination index.