Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences, annealing temperature, and polymerase chain reaction (PCR) product size used for methylation-sensitive restriction analysis (MSRA).
| Gene | Amplicon Length (bp) | Sequence of Primers (5′ → 3′) F—Forward; R—Reverse |
Annealing Temperature (°C) | UCSC RefSeq Gene Accession NM (Human GRCh37/hg19 Assembly) |
|---|---|---|---|---|
| RARB | 295 | F: CTCGCTGCCTGCCTCTCTGG | 58.4 | NM_016152 |
| R: GCGTTCTCGGCATCCCAGTC | chr:3 | |||
| PTEN | 214 | F: CAGCCGTTCGGAGGATTATTC | 61.1 | NM_000314 |
| R: GGGCTTCTTCTGCAGGATGG | chr:10 |
