Table 3.
Primer sequences, annealing temperature, and PCR product size used for quantitative real-time PCR (qPCR).
| Gene | Amplicon Length (bp) | Sequence of Primers (5′ → 3′) F—Forward; R—Reverse |
Annealing Temperature (°C) |
|---|---|---|---|
| RARB | 92 | F: TTCAAGCAAGCCTCACATGTTTCCA | 58.4 |
| R: AGGTAATTACACGCTCTGCACCTTTAG | |||
| PTEN | 330 | F: CGAACTGGTGTAATGATATGT | 50.0 |
| R: CATGAACTTGTCTTCCCGT | |||
| DNMT1 | 100 | F: ACCGCCCCTGGCCAAAGCCATTG | 60.0 |
| R: AGCAGCTTCCTCCTCCTTTATTTTAGCTGAG | |||
| CDKN1A | 103 | F: GCTCAGGGGAGCAGGCTGAAG | 60.0 |
| R: CGGCGTTTGGAGTGGTAGAAATCTGT | |||
| TP53 | 120 | F: TAACAGTTCCTGCATGGGCGGC | 64.0 |
| R: GGACAGGCACAAACACGCACC | |||
| H3F3A | 76 | F: AGGACTTTAAAACAGATCTGCGCTTCCAGAG | 65.0 |
| R: ACCAGATAGGCCTCACTTGCCTCCTGC | |||
| RPLP0 | 69 | F: ACGGATTACACCTTCCCACTTGCTGAAAAGGTC | 65.0 |
| R: AGCCACAAAGGCAGATGGATCAGCCAAG | |||
| RPS17 | 87 | F: AAGCGCGTGTGCGAGGAGATCG | 64.0 |
| R: TCGCTTCATCAGATGCGTGACATAACCTG |