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. 2018 Oct 24;10(4):200. doi: 10.3390/pharmaceutics10040200

Figure 3.

Figure 3

Representative images of Calcein stained cells (green) seeded on LFAMs (auto-fluorescent in red) with the 13 different protocols suggested by Design of Experiment (DoE) (a) (1: 20 RPM, 30 min, intermittent, 1000 µL; 2: 20 RPM, 120 min, intermittent, 1000 µL; 3: 10 RPM, 60 min, intermittent, 400 µL; 4: 10 RPM, 120 min, intermittent, 400 µL; 5: 5 RPM, 30 min, intermittent, 400 µL; 6: 5 RPM, 90 min, intermittent, 1000 µL; 7: 20 RPM, 60 min, continuous, 400 µL; 8: 20 RPM, 90 min, continuous, 400 µL; 9: 10 RPM, 30 min, continuous, 1000 µL; 10: 10 RPM, 60 min, continuous, 1000 µL; 11: 10 RPM, 120 min, continuous, 400 µL; 12: 5 RPM, 30 min, continuous, 400 µL; 13: 5 RPM, 120 min, continuous, 1000 µL) and the Standard Protocol (b) (SP: 70 RPM, 120 min, continuous, 1000 µL). Scale bar = 500 µm. Semi-quantitative evaluation of Calcein-stained cells on the surface of LFAMs (c).