KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-human HMGN1 dilution 1:1000 | Bethyl | A302-363A |
| Rabbit anti-human BCL6 dilution 1:1000 | Cell Signaling Technology | 14895S |
| Rabbit anti-human beta-Actin dilution 1:1000 | Sigma | SAB5500001 |
| Rabbit anti-human CD79A dilution 1:1000 | Santa Cruz | sc-25604 |
| Rabbit anti-human Lyn dilution 1:1000 | Santa Cruz | sc-15 |
| Mouse anti-human Syk dilution 1:1000 | Santa Cruz | sc12-40 |
| Rabbit anti-human H3K27me3 (ChIP) | Cell Signaling Technology | 9733 |
| Rabbit anti-human H3K4me3 (ChIP) | Abcam | ab8580 |
| Rabbit anti-human H3K27acetyl (ChIP) | Abcam | ab4729 |
| Rabbit IgG | Cell Signaling Technology | 2729 |
| Rabbit anti-human H3K27acetyl dilution 1:500 (Flow) | Abcam | ab4729 |
| Rabbit anti-human phospho-Akt (Ser473) dilution 1:500 (Flow) | Cell Signaling Technology | 4060 |
| Goat anti-Rabbit IgG Alexa Fluor 555 dilution 1:1000 | Invitrogen | A21428 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant mouse IL3 | Gold Biotechnology | 1310-03-2 |
| B cell methylcellulose with IL7 | Stem Cell Technologies | M3630 |
| Doxycycline hydrochloride | Fisher Scientific | BP26531 |
| 5-Bromouridine | Sigma | B7166 |
| Trizol | Life Technologies | 15596018 |
| ERCC Spike-in | Thermo Fisher/Ambion | 4456740 |
| 4-Thiouridine | Sigma | T4509 |
| Puromycin dihydrochloride | Gold Biotechnology | P-600 |
| Polybrene | Santa Cruz | sc-134220 |
| Lipofectamine 2000 | Life Technologies | 11668500 |
| RNase | Sigma | R6513 |
| Critical Commercial Assays | ||
| Qubit RNA BR assay kit | Life Technologies | Q10210 |
| Quantifluor RNA System | VWR | PAE3310 |
| Agilent RNA 6000 Nano Kit | Agilent | 5067-1511 |
| SimpleChIP enzymatic chromatin IP kit | Cell Signaling Technology | 9005 |
| DNA-free DNA removal kit | Invitrogen | AM1906 |
| High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | 4368814 |
| Power SYBR Green PCR master mix | Applied Biosystems | 4367659 |
| Deposited Data | ||
| Raw and analyzed RNA-seq and ChIP-seq data | This paper | GEO: GSE121071 https://www.ncbi.nlm.nih.gov/geo/ |
| Experimental Models: Cell Lines | ||
| Human: Nalm6 | DSMZ | ACC 128 |
| Mouse: FL5.12 | Laboratory of Anthony Letai | N/A |
| RPE with MMCTof chr21 | This paper and Lane et al.,2014 | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Ts1Rhr B6.129S6-Dp(16Cbr1-Fam3b)1Rhr/J, backcrossed to C57BL/6J > 10 generations | Jackson Laboratory | 005383 |
| Mouse: HMGN1-OE, backcrossed to C57BL/6J > 10 generations | Laboratory of Michael Bustin | N/A |
| Mouse: C57BL/6J wild-type | Jackson Laboratory | 0000664 |
| Oligonucleotides | ||
| Primers for RT-PCR and ChIP-PCR, see Table S1 | This paper | N/A |
| Recombinant DNA | ||
| Plasmid: pCW57.1 (TRE-gateway; PGK-rtTA-2A-puro) | This paper | Addgene 41393 |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Morpheus | N/A | https://software.broadinstitute.org/morpheus/ |
| Gene Set Enrichment Analysis | Subramanian et al., 2005 | https://www.broadinstitute.org/gsea/ |
| SlamDunk v0.2.4 | Herzog et al., 2017 | https://github.com/t-neumann/slamdunk |
| ROSE | Whyte et al., 2013 | https://bitbucket.org/young_computation/rose |