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. 2018 Mar 20;10(1):43–59. doi: 10.1007/s13238-018-0512-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fir1 is required for missing-tissue regeneration. (A) Fir1(RNAi) animals did not form blastemas after amputation (arrowheads, n > 100). Scale bars, 500 μm. (B) Colorimetric WISH of Fir1(RNAi) middle pieces following 8 days regeneration for markers of porcupine (intestine) and PC2 (neurons). Scale bars, 200 μm. (C) The regeneration phenotypes after different injury types of Fir1(RNAi) animals. The injury included: a large incision, excision of a wedge of lateral tissue, removal of head tips and removal of eyes. ovo is an eye progenitor marker. For each surgical types, n = 6 animals. Scale bars, 500 μm