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The construction and characteristics of the donor DNA, and the scheme for the intraspecies and interspecies transformation experiments. Asterisks indicate the SNV in the rpoB gene encoding rif resistance. The rpoB gene was placed within a circular chromosome (diagram not-to-scale) . Red arrows indicate the primers used to generate the RifR Z6793 donor. The recipient Mc strain MC58 is illustrated as a diplococcus. Nl, N. lactamica.
