Fig. 3.

Twitching motility and PilQ secretin phenotypes in PAK05353 (PA14_66580 mutant). (a) Subsurface twitching motility-mediated interstitial biofilm expansion at agar/plastic interface after 48 h of incubation at 37 °C for PAK and clean deletion of PA14_66580 in PAK (PAK05353) is presented as the mean (±sem) surface area (mm²) normalized against the wild-type as obtained from three independent experiments performed in triplicate. A two-tailed Student’s t-test was used; *P<0.0005 compared to the wild-type. (b) Immunoblot of PilQ from whole cell preparations of strains PAK, PAK05353 and PAKpilQ obtained from overnight (20 h) confluent lawns grown at 37 °C on LB agar plates. RNAP was used as a loading control.