FIG 7.

CAA cells induce angiogenesis in vivo in an IL-8-depednent manner. (A and D) FFPE tissues were prepared from the indicated tumors, and immunohistochemistry was carried out with anti-CD34 antibody. Scale bars, 50 µm. (B and E) Histograms show the average numbers of CD34⁺ microvessels observed in five different fields from the different tumors. Error bars represent means ± the SD. *, P ≤ 0.0013. (C and F) Same as in Fig. 3H and 6B. The numbers below the bands indicate the corresponding protein expression levels relative to GAPDH. The levels of phosphorylated proteins were normalized against the total amount of the respective nonphosphorylated proteins. (G) SFM or SFCM from the indicated adipocytes were collected and were added independently on HUVEC cells plated on Matrigel (96-well plate), and the differentiation into capillary-like structures was assessed after 5 h of incubation. Representative photographs of HUVEC cavities are shown. Scale bars, 30 µm. (H) A histogram shows the average numbers of microvessels counted in five different fields. Error bars represent means ± the SD. *, P ≤ 1.19 × 10⁻⁶.