FIG 4.

Leaderless FMDV is unable to suppress SG formation. (A) LFPK αvβ6 cells were infected with FMDV-A12 or leaderless FMDV-A12 (LLV) and fixed at 4 hpi, and immunofluorescence staining was performed for the viral capsid protein VP1 (green) and the SG marker G3BP1 (red). (B) Quantification of data from panel A in which the percentages of SG-positive infected cells and the numbers of SGs per cell were determined in at least 50 cells. A one-tailed t test was used to determine statistical significance. **, P < 0.01; ***, P < 0.001.