FIG 1.

Reduced EBV replication in HPV-immortalized rafts following EBV infection. (A) Schematic of EBV infection of HPV-immortalized human tonsillar epithelial (HPV-pos HTE) cells in raft culture. (B) HTE raft DNA harboring either episomal or integrated HPV was analyzed for exonuclease digestion resistance by qPCR. DNA from HPV-infected 293TT cells served as an episomal control. qPCR was used to detect HPV16 DNA (E6), mitochondrial DNA (Mito), and 18S ribosomal DNA. (C) EBV genome copy numbers per hCRP copy number in primary HTE (HPV-neg; n = 6) and HPV-pos HTE rafts harboring episomal (n = 3) or integrated (n = 6) HPV genomes were quantified by qPCR. Shown are the average values and standard errors of the means. *, P < 0.05 relative to the results for HPV-neg HTE rafts. (D) EBV genome copy numbers per hCRP copy number in NOK rafts were quantified by qPCR. Shown are the average values and standard errors of the means. *, P < 0.05 relative to the results for EBV. Rafts were infected in the presence or absence of acyclovir (ACV; 50 μg/ml).