FIG 1.

IFITM3 mutants used here. (A) IFITM3 mutants used in this study. Mutants that could not be reliably detected upon transient DNA transfection of HEK293T cells were excluded from our study and are not presented here. FACS, fluorescence-activated cell sorter. (B) The intracellular localization of these mutants was analyzed by confocal microscopy, and representative pictures are displayed. Of note, this analysis is meant to provide a qualitative assessment of the intracellular distribution of the different IFITM3 mutants and does not reflect their relative expression levels, which instead were quantified by WB.