FIG 6.

IFITM3 mutants modulate the extent of accumulation of CD63-positive vesicles as well as MVBs in cells, but these properties do not correlate with their antiviral behaviors. (A and B) Cells expressing or not expressing a short list of IFITM3 mutants were analyzed by confocal microscopy using the endosomal marker CD63. (C) Same as above, but cells were analyzed by electron microscopy. A representative picture of a cell expressing WT IFITM3 is displayed, while the number of scored MVB-positive cell sections in the different mutants is presented in the graph (please note the different scale). (D and E) Correlative analyses, as indicated. The graphs present data obtained in 2 independent experiments (electron microscopy) or 3 independent experiments (immunofluorescence analysis) with over 100 scored cells under each condition.