FIG 3.

(A and B) Production of gC and ICP27 in virus-infected cells. 4T1 (A) and MDA-MB-231 (B) cells were mock infected or infected with the indicated viruses at 5 PFU per cell. At 12 h postinfection, cells were harvested and subjected to Western blot analysis with antibodies against gC, ICP27, and β-actin. (C and D) Effects of viral infection on eIF2α phosphorylation. 4T1 (C) and MDA-MB-231 (D) cells were mock infected or infected with viruses. At 12 h postinfection, lysates of cells were subjected to immunoblotting analysis with antibodies against eIF2α, phosphorylated eIF2α (Ser51), γ₁34.5, and β-actin. The data are representative of those from three independent experiments.