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. 2018 Oct 9;28(2):290–306. doi: 10.1093/hmg/ddy351

Figure 5.

Figure 5

Relative Complex IV activity and quantity in fibroblasts of Mother and affected Siblings. (A) Kinetic comparison of Complex IV activities in protein extracts from Siblings 1 and 2 and Mother fibroblasts. (B) Endpoint assay analyzing Complex IV activity in protein extracts (50 μg) from Sibling 2 and Mother fibroblasts. The latter was serially diluted from 50 to 6.25 μg and used as a reference control. Corresponding densitometric analysis is shown below. (C) Complex IV protein quantity analysis in protein extracts (20 μg) from Siblings 1and 2 and Mother fibroblasts. The latter was serially diluted from 20 to 2.5 μg and used as a reference control. Corresponding image densitometry analysis is shown below.