Figure 1.
A schematic of the pipeline for performing multiplexed, real-time, digital loop-mediated isothermal amplification (LAMP) using only commercially available and/or open source components. Microfluidic chips and reagents (e.g., primers, enzymes, buffer composition) can be purchased commercially. Multiple instrument configurations can be used to capture results, e.g., a customized real-time instrument (instructions for building publicly available30) or any commercial microscope. Data analysis is automated using a MATLAB script (Supporting Information, S-I).