Figure 3.

Fraction of signal attributable to neutral loss observed in spectra derived from 5749 monophosphorylated pS and pT tryptic peptides using ion trap CID fragmentation (A). The extent of neutral loss correlates with proton mobility but does not fully explain the observed behavior. The observed fraction of signal intensity attributable to H₃PO₄ neutral loss, as a function of the proximity of the phosphorylation site to both peptide termini, is displayed in part B. Reprinted by permission from Springer. J. Am. Soc. Mass Spectrom., Large-Scale Examination of Factors Influencing Phosphopeptide Neutral Loss during Collision Induced Dissociation. Brown, R., Stuart, S. S., Houel, S., Ahn, N. G., Old, W. M. Vol. 26, 2015, pp. 1128–1142 (ref (40)). Copyright 2015.