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EThcD fragmentation spectrum of a doubly phosphorylated peptide. Complete sequence coverage is achieved while neutral losses are minimal, enabling unambiguous localization of both phosphosites. Reproduced from Frese, C. K.; Zhou, H.; Taus, T.; Altelaar, A. F. M.; Mechtler, K.; Heck, A. J. R.; Mohammed, S. J. Proteome Res.2013, 12, 1520–1525 (ref (95)). Copyright 2013 American Chemical Society.
