Table 3.
Gene expression analysis performed in mosquito lysates by direct qPCR and matched purified eluates after nucleic acid extraction (n = 4 susceptible vs n = 4 resistant). P values were calculated by the pair wise fixed reallocation randomization test
| Gene (Detox assay) | Direct qPCR in lysates | qPCR in matched nucleic acid extracted eluates | ||
|---|---|---|---|---|
| Fold change (95% CI) | P-value | Fold change (95% CI) | P-value | |
| CYP6P3 (A) | 4.68 (2.67–8.81) | 0.001 | 4.10 (3.35–5.23) | 0.020 |
| CYP6M2 (A) | 4.57 (3.18–7.19) | 0.002 | 2.70 (2.18–3.40) | 0.022 |
| CYP9K1 (B) | 4.66 (3.40–7.70) | 0.002 | 4.45 (4.12–4.80) | <0.001 |
| CYP6P4 (B) | 2.29 (1.31–3.71) | 0.018 | 1.67 (1.33–2.11) | <0.001 |
| CYP6Z1 (C) | 2.98 (2.47–3.84) | 0.009 | 1.85 (1.44–2.22) | <0.001 |
| GSTE2 (C) | 13.6 (9.64–18.4) | 0.008 | 9.36 (7.96–11.9) | <0.001 |
| CYP6P1 (D) | 1.32 (0.968–1.90) | 0.060 | 1.64 (0.752–3.57) | 0.215 |
| CYP4G16 (D) | 2.81 (1.61–4.26) | 0.008 | 1.91 (1.45–2.46) | <0.001 |