Table 4.

Cost comparison of triplex TaqMan vs singleplex SYBR Green approach. This is a rough cost estimation for measuring the gene expression levels of eight detox genes and one normaliser gene in 12 samples on a standard 96-well plate format

Consumables and reagents	TaqMan triplex RT-qPCR	SYBR Green singleplex RT-qPCR
96-well plastic plate and optical film	1 × 6.50 € = 6.50 €	3 × 6.50 € = 19.50 €
Mastermix	1 × 25.00 € = 25.00 €	2.25 × 25.00 € = 56.25 €
Primers	1 × 0.50 € = 0.50 €	2.25 × 0.50 € = 1.13 €
TaqMan Probes	1 × 10.00 € = 10.00 €	–
Total cost	42.00 €	76.88 €