Fig. 6.

The colon myeloid immune compartment of Rag1^−/− shifted towards a more pro-inflammatory phenotype after intestine-specific sympathectomy. a Gating strategy. b Frequency of parent (%) and absolute cell number of different myeloid populations (CD45⁺: CD45⁺ cells, Mo: monocytes, DC: dendritic cells) in the colon of Rag1^−/− mice 2 weeks after sympathectomy (sx) or a sham operation (sham). c Frequency of parent (%) of immature monocytes and resident macrophages present in the colon. d Absolute cell numbers of immature monocytes and resident macrophages present in the colon. e mRNA expression levels of interleukin (IL)-1β, IL-6, IL-10, IL-12 and tumour necrosis factor (TNF)-α in sorted immature monocytes and resident macrophages from the colon. We normalized expression for reference genes β-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). N = 10–11 mice pooled per 2–3 animals for analysis. Data is expressed as median and individual data points. We tested for statistical significant differences with a Mann-Whitney U test. P-value < 0.05 was considered significant