Fig. 4.

TFF1 is a target gene of miR-632. a Recombinant TFF1 protein rescued TFF1 expression inhibited by miR-632-mimic in AGS and BGC823 cells. B The expression of MMP9 (a) and CD34 (b) with recombinant TFF1 treatment in miR-632-mimic-transfected AGS and BGC cells. c Schematic diagram showing the miR-632-mediated co-culture system for angio-tube formation assays with or without recombinant TFF1 in GC cells. d Recombinant TFF1 reversed tube formation mediated by miR-632 (left panels). The histograms present the total tube length (mean ± SD) from three random fields at high magnification (right panel). e Schematic diagram showing the miR-632-mediated co-culture system used for endothelial cell Transwell assays with or without TFF1 recombinant protein in GC cells. f TFF1 recombinant protein reversed endothelial cell recruitment mediated by miR-632 (left panels). The histograms present the cell numbers (mean ± SD) from three random fields at high magnification (right panels). G Schematic diagram showing miR-632 and potential binding regions in the 3’UTR of TFF1 (a). (b) Relative luciferase activity of the TFF1–3’UTR reporter (left panel) and mutated-3’UTR reporter (right panel) in cells treated with miR-632-mimic compared with the control. The experiments were performed at least three times independently. *P < 0.05; **P < 0.01