Fig. 2.

Deletion of Bmal1 in joint mesenchymal cells renders fibroblast-like synoviocytes (FLS) and chondrocytes arrhythmic. a Cultured and purified FLS and femoral head tissue from Col6a1-Bmal1^−/− and wild-type animals (on a PER2::luc background) were placed under photomultiplier tubes to record bioluminescence (representative of n = 3/genotype). b Cultured FLS were harvested every 4 h for RNA extraction, and clock gene expression was profiled. Values are made relative to expression in wild-type cells at time point 0. c Clock gene expression in FLS (CD45⁻CD90.2⁺) sorted from the limbs of male and female mice (9–13 weeks) at ZT6 and ZT18 (n = 4–6). Two-way analysis of variance and post hoc Bonferroni correction were performed