Table 1.
Properties of the synthetic L. sericata AMPs
| AMPa | Sequence | Size | MWb | pIb | Chargeb | Gc |
|---|---|---|---|---|---|---|
| LS-sarcotoxin | GWLKKIGKKIERVGQHTRDATIQTIGVAQQAANVAATLK-NH2 | 39 | 4199.86 | 11.63 | +6.1 | −0.321 |
| all-d LS-sarcotoxin | d-(GWLKKIGKKIERVGQHTRDATIQTIGVAQQAANVAATLK)-NH2 | 39 | 4199.86 | 11.63 | +6.1 | −0.321 |
| LS-stomoxyn | GFRKRFNKLVKKVKHTIKETANVSKDVAIVAGSGVAVGAAM-NH2 | 41 | 4326.13 | 11.72 | +8.1 | 0.059 |
| all-d LS-stomoxyn | d-(GFRKRFNKLVKKVKHTIKETANVSKDVAIVAGSGVAVGAAM)-NH2 | 41 | 4326.13 | 11.72 | +8.1 | 0.059 |
a
‘all-d’ signifies l-amino acids were replaced by the corresponding d-amino acids.
b
The AMP properties molecular weight (MW), isoelectric point (pI) and net charge at pH 7 (Charge) were calculated using software provided at http://pepcalc.com/.
c
G, GRAVY score, total hydropathy values of all the amino acids divided by the size.96