Table 5.
Fold decrease in MIC compared with CAMHBa |
|||||||||
---|---|---|---|---|---|---|---|---|---|
LS-sarcotoxin |
LS-stomoxyn |
||||||||
Test strainb | Phenotype | N | C | S | S + N | N | C | S | S + N |
E. coli | S | 1 | 1 | 16 | 128 | 1 | 1 | 64 | 256 |
E. coli | MEMR | 1 | 1 | 32 | 128 | 1 | 0.5 | 128 | 256 |
K. pneumoniae | S | 1 | 1 | 32 | 32 | 0.5 | 0.5 | 128 | 256 |
K. pneumoniae | MEMR | 0.5 | 1 | 8 | 32 | 0.5 | 0.5 | 64 | 256 |
A. baumannii | S | 1 | 1 | 16 | 128 | 0.5 | 0.5 | 32 | 256 |
A. baumannii | MEMR | 1 | 1 | 16 | 128 | 1 | 0.5 | 64 | 256 |
P. aeruginosa | S | ND | ND | 0.5 | 0.25 | 0.5 | 0.5 | 4 | 4 |
P. aeruginosa | MEMR | ND | ND | 0.25 | 0.25 | 1 | 0.5 | 0.5 | 0.5 |
E. coli | CSTR | ND | ND | 16 | 32 | ND | ND | 32 | 64 |
K. pneumoniae | CSTR | ND | ND | 8 | 16 | ND | ND | 16 | 64 |
ND, not determined.
MICs were determined in CAMHB, CAMHB adjusted to 150 mM NaCl (N) or 1.25 mM CaCl2 (C), CAMHB supplemented with 10% human serum (S) and CAMHB supplemented with 10% human serum and adjusted to 150 mM NaCl (S + N). Fold changes in MIC values were calculated with respect to the MIC values obtained in CAMHB.
MIC values were determined for the colistin/meropenem-susceptible (S) strains E. coli ATCC 25922, K. pneumoniae DSM 30104, A. baumannii ATCC 19606, P. aeruginosa ATCC 27853, the meropenem-resistant (MEMR) strains E. coli RKI 131/03, K. pneumoniae RKI 93/10, A. baumannii RKI 19/09, P. aeruginosa RKI 93/12 and the colistin-resistant (CSTR) strains E. coli RKI 6A-6 and K. pneumoniae RKI 19/16.