Fig. 1.

Disruption of Cmah gene in NSG strain mouse using the CRISPR/Cas9 system. a Schematic of CRISPR sgRNA guides to disrupt Exon 6 of CMAH gene. b Sequence alignment of the wild type and the deletion allele. The guide sequences are shown in red and the protospacer adjacent motif (PAM) sequences are in green. c The founder number 2, containing a prominent deletion band, was bred to segregate the mutant allele. Genotyping PCR of F1 mice showing mixture of wild type (line 2 and 6) and with the deletion bands (1, 3, 4, 5). d Genotyping of the homogygous NSG-cmah^−/− line established after 4 generations of breeding of the founder #2 (n = 10)