Fig. 3.

Effects of the cmah^−/− background on human immune cells expansion and activation after HSPC transplantation. At 3 and 6 months post CD34⁺ cell transplantation, the frequency of circulating human lymphocyte subsets were analyzed. a and b Representative plots and gating strategies for human B-cell and T-cell enumeration. c Individual mouse NSG-cmah^−/− (open symbol, n = 11) and NSG wild type mice (closed symbol, n = 10) and means with SEM are shown for human B and T cells in mouse peripheral blood. Naïve phenotype of CD3⁺CD45RA⁺ T-cells exhibited better persistence at 6 months of age in the NSG-cmah−/− strain. Lower proportions of mature CD19⁺IgD⁺ B cells in peripheral blood of cmah^−/− mice at 6 months of age, as well as a lower frequency of CD22 B-cell expression at 3 and 6 months of age, were also observed. P values were determined with Kruskal-Wallis test and Dunn’s multiple comparisons tests (*) P values determined by Mann-Whitney test (#), and paired t-test (@) are shown