Fig. 6.

Effects of the cmah^−/− background on human cell responses to HIV-1 infection in bone marrow. a Human differentiated T cells, b precursors and mature B cells, c myeloid lineage cells FACS analyses. Mice bone marrow were reconstituted with comparable proportions of human CD45⁺ cells; HIV-1 mediated depletion of T cells was more pronounced in NSG-cmah^−/− animals compared to NSG wt (n = 4–5). HIV-1 infection increased the relative proportion of B cell precursors and mature B-cells (CD20⁺) and the proportion of CD14⁺ macrophages compared to NSG mice. Individual mouse and means with SEM are shown. P values were determined with Kruskal-Wallis test and Dunn’s multiple comparisons tests (*) and Mann-Whitney test (#). P ≤ 0.05 were considered significant. The gating strategies are shown in Additional file 6: Figure S6.