Fig. 3.
Effector memory T (TEM) cells and cytokines regulate the phenotype of fibroblast-like synoviocytes (FLS). KCa1.1α (a) and major histocompatibility complex (MHC) class II molecule (b) plasma membrane expression levels by collagen-induced arthritis (CIA)-FLS cultured for 72 h in the conditioned medium of TEM cells that were antigen-stimulated in the presence or absence of ShK-186. Data are presented as mean ± SEM (n = 6–9 CIA-FLS donors). c KCa1.1α protein expression by rheumatoid arthritis (RA)-FLS, as determined by flow cytometry, following 24 h of stimulation with recombinant cytokines. Data are presented as mean ± SEM (n = 7–9 RA-FLS donors). d CIA-FLS invasion through Matrigel-coated Transwells toward the conditioned medium of TEM cells that were antigen-stimulated in the presence or absence of ShK-186. Data are presented as mean ± SEM (n = 3 CIA-FLS donors). *p < 0.05, **p < 0.01