Table 2.
Targets, primers and amplicon size for the PCR-testing from ticks and EDTA-blood
| Target sequence | Designation | Sequence (5'-3') | Amplicon length (bp) | Reference |
|---|---|---|---|---|
| Bartonella spp. 16S rDNA, 1st round | A-proteo | AGAGTTTGATC(AC)TGGCTCAGA | 1210 | [44] |
| r-Alpha-sh | GTAGCACGTGTGTAGCCCA | |||
| Bartonella spp. 16S rDNA, 2nd round | Bart | CACTCTTTTAGAGTGAGCGGCAA | 990 | [44] |
| r-BH | CCCCCTAGAGTGCCCAACCA | |||
| Bartonella 16S-23S ITS region | 325s | CTTCAGATGATGATCCCAAGCCTTCTGGCG | various Bartonella spp., ~500 bp | [45] |
| 1100as | GAACCGACGACCCCCTGCTTGCAAAGCA | |||
| Bartonella spp. rpoB | prAPT0244 | GATGTGCATCCTACGCATTATGG | 406 | [51] |
| prAPT0245 | AATGGTGCCTCAGCACGTATAAG | |||
| Anaplasma spp. 16S rDNA 1st round | ge3A | CACATGCAAGTCGAACGGATTATTC | 932 | [48] |
| ge10r | TTCCGTTAAGAAGGATCTAATCTCC | |||
| Anaplasma spp. 16S rDNA 2nd round | ge9f | AACGGATTATTCTTTATAGCTTGCT | 546 | [48] |
| ge2 | GGCAGTATTAAAAGCAGCTCCAGG | |||
| C. burnetii IS1111 | CB_S4k | GAAACGGGTGTTGAATTGTTTG | 290 | [47] |
| CB_A2k | ATCACCAATCGCTTCGTCCCGGT | |||
| Rickettsia spp. 23S-5S ITS region | 23S for | GATAGGTCGGGTGTGGAAGCAC | various Rickettsia spp., ~500 bp | [46] |
| 23S rev | GGGATGGGATCGTGTGTTTCAC | |||
| Leptospira spp. LipL32 | LipL32-270F | CGCTGAAATGGGAGTTCGTATGATT | 423 | [49] |
| LipL32-692R | CCAACAGATGCAACGAAAGATCCTTT | |||
| Borrelia spp. 16S rDNA | 16S FW | GGCTTAGAACTAACGCTGGCAGTGC | 552 | [50] |
| 16S RV | CCCTTTACGCCCAATAATCCCGA |