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. 2019 Jan 7;52:1. doi: 10.1186/s40659-018-0209-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Compound 1 induced apoptosis in CAL-27 cell line in a dose-dependent manner. a–c Flow cytometric analysis of the apoptosis induced by the compound 1 at IC₅₀ and IC₇₀ concentrations. An increased number of apoptotic cells were observed after treatment as compared to control. d Graphical representation of the cells in different phases of apoptosis after 48 h of treatment. Statistical significance were expressed as **p < 0.01 and ***p < 0.001 as compared to the untreated control