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. 2018 Dec 11;20(1):e46224. doi: 10.15252/embr.201846224

Figure EV2. QRD is required to localize TIAR in GMGs.

Figure EV2

  1. Scheme of TIAR mutants. All TIARr plasmids contain a silent point mutation at amino acid V244 and R245, which renders the corresponding mRNA resistant to siRNA S62. In TIARr‐dQRD, the C‐terminus of TIAR (amino acids 284–375) was deleted by insertion of a thymidine residue before N284, thereby introducing a stop codon. TIARr‐RRM123m has the following point mutations: Y50A, F52A, F100A, Y140A, F142A, Y242A, and Y244A, as indicated by asterisks.
  2. HeLadox‐YFP‐TIARr, HeLadox‐YFP‐TIARr‐RRM123m, and HeLadox‐YFP‐TIARr‐dQRD cells were transfected with control or TIAR siRNAs, and cultured in the absence or presence of 200 μM doxycycline for 24 h. Cells were treated with 0.4 μM APH for an additional 16 h, fixed with methanol, and analyzed by IF microscopy after staining with anti‐p(S10)‐H3 antibody and DAPI.