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A, B
Similar extent of expression of all three DBN1 versions (WT, S142A, S142D) were observed in both WT (A) and DBN1 KO (B) cells (around 10‐fold compared to WT cells). Note that the WT and WT with DBN1
WT, DBN1
S142A, and DBN1
S142D samples were analyzed on the same blotting membrane. The DBN1/GAPDH ratios were densitometrically measured. The results are mean ± SD of two independent experiments. *P ≤ 0.05. One‐way ANOVA.
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C
DBN1 could not be detected in cilia. Antibody localization of DBN1 in ciliated RPE1 cells. CEP164 was used as a marker for the mother centriole and ARL13B for the cilium. The line scan on the right shows signal distribution along the cilium in the boxed on the left area for CEP164, ARL13B, and DBN1 (green).
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D
TetON‐DBN1‐GFP was expressed in ciliating RPE1 cells upon addition of Dox. The line scan on the right reflects ARL113B, CEP164, and DBN1‐GFP (green) intensity along the cilium shown in the boxed area on the left.
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E
DBN1‐GFP and F‐actin co‐localized in RPE1 cells during ciliogenesis. The intensities of both signals (right) were measured along the white line in the merge image on the left of ciliating RPE1 TetON‐DBN1‐GFP cells.
Data information: (C, D and E) Scale bars: 5 μm.
